Setup for Brightfield Transmitted with Phase Contrast and Fluorescence Reflective studies
This Zeiss Axiovert 200M Inverted Fluorescence Motorized Phase Contrast Microscope is in good working order with all aspects tested and working fully.
Microscope and optics have been professionally cleaned under a class 10,000 clean room hood.
Please see the exact video and photos below.
Testing we performed:
The Zeiss Axiovert 200M Inverted Fluorescence Motorized Phase Contrast Microscope passed all tests and was found to be fully functional:
Tested mechanical XY stage to limits: PASSED
Tested mercury lamp and source fully: PASSED
Tested halogen lamp and source fully: PASSED
Tested all control buttons and switches: PASSED
Tested the port splitter to switch between all ports: PASSED
Tested connection from computer to camera: PASSED
Tested Z-axis adjustment and focus: PASSED
Tested live feed to computer: PASSED
Tested filters and prisms: PASSED
Tested all components of both light paths: PASSED
Tested all motorized components including objective nosepiece, filter cube turret, Z-axis, light path selector and fluorescence shutter: PASSED
Tested full control of every motorized component via laptop: PASSED
During testing this Zeiss Axiovert 200M Inverted Fluorescence Motorized Phase Contrast Microscope was found to have no issues.
A clear image was produced through the eyepieces as well as through the camera port.
All of the mechanical and Motorized components moved freely and the test sample was able to be focused without excessive effort.
System consists of:
Zeiss Axiovert 200M Inverted Microscope
Binocular tube head
(2) W-PI 10x/23 Eyepieces
Prisms / Condenser:
Motorized Condenser NA 0.55 26mm
Prisms: Ph1, Ph2
Transmitted light Illumination Tower
Motorized integrated shutter
HAL100 12V 100W Halogen Lamp with built-in power supply
HBO100 100W Mercury Lamp with ebq100 power supply
Fluorescence Turret / Cubes:
5-position Motorized Refection Turret
Green- EX: 545/30nm DM: 565nm EM: 610/75nm
TRITC- EX: 535nm DM: 570nm EM: 595lp
Blue/Violet- EX: 436/20nm DM: 455lp EM: 480/40nm
32x/0.40 ACHROSTIGMAT Ph1
Photo / Port Splits:
Left 100% or 60%Left 40% Head or Head 100%
100% Eye or 100% Camera port
AmScope MU1003 10.0 MP CMOS Digital Camera
Objective Turret, Filter Cube Turret, Z-axis Focus, Halogen Lamp/Brightness, Fluorescence Shutter, and light path switching
Computer and Software:
Dell Latitude i5 Laptop running Windows 7 Pro with AmScope and MicroManager software
Communication and Power Cables:
(2) Standard 115V Power Cords
M to F NULL RS232 9 pin to Plugables adapter
USB3.0 Communication Cable
This Zeiss Axiovert 200M Inverted Fluorescence Motorized Phase Contrast Microscope is currently set up for Brightfield transmitted with Phase Contrast and Fluorescence reflective studies.
This Zeiss Axiovert 200M Inverted Fluorescence Motorized Phase Contrast Microscope is intended for Biological studies of cells and slide samples.
The AmScope CMOS digital colored camera is capable of taking detailed digital images. The Zeiss system includes a Dell Latitude i5 Laptop to control the AmScope and MicroManager software.
This includes control of all motorized components such as the objective turret, filter cube turret, z-axis focus, halogen lamp/brightness, fluorescence shutter and port switching.
This video begins with a full front view of the Zeiss Axiovert 200M Inverted Fluorescence Motorized Phase Contrast Microscope powered up and running.
The camera pans from right to left for a closer look. Next we see the live feed with a demonstration using the mechanical option for focus.
We see the fluorescence lighting with the visible eye. A final front view of the Zeiss Axiovert 200M Inverted Fluorescence Motorized Phase Contrast Microscope will end this video.
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