Here is a Zeiss Axioplan 2ie Mot Fluorescence Motorized Phase Contrast Microscope
Setup for Brightfield with Phase Contrast and Fluorescence
Current models is Axio Scope A1
This Zeiss Axioplan 2ie Mot Fluorescence Motorized Phase Contrast Microscope is in good working order with all aspects tested and fully working.
Microscope optics have been cleaned professionally by our technician under a class 10,000 clean room hood.
Please see the exact video and photos below.
Testing we performed:
The Zeiss Axioplan 2ie Mot Fluorescence Motorized Phase Contrast Microscope passed all tests and was found to be fully functional:
Tested halogen lamp and source fully: PASSED
Tested fluorescence and source fully: PASSED
Tested transmission halogen light path: PASSED
Tested reflective fluorescence light path: PASSED
Tested objective for clarity: PASSED
Tested port splitter to split between camera and eyepieces: PASSED
Tested motorized condenser and prisms: PASSED
Tested motorized stage to limits: PASSED
Tested motorized Z-axis: PASSED
Tested motorized nosepiece: PASSED
Tested motorized fluorescence turret: PASSED
Tested connection from camera to laptop: PASSED
Tested control of motorized stage virtually: PASSED
Tested live feed for clarity: PASSED
Tested all filter slides: PASSED
Tested all switches and adjustment knobs to be working fully: PASSED
During testing and cleaning this Zeiss Axioplan 2ie Mot Fluorescence Motorized Phase Contrast Microscope was found to have no issues.
A clear image was produced through the eyepieces as well as through the camera port.
All of the mechanical components moved freely and the test sample was able to be focused without excessive effort.
System consists of:
Zeiss Axioplan 2ie Mot Upright Microscope
(2) 10X/21 Eyepieces
Prisms and Condensers:
Condenser Lens 0.9
6-position Motorized Condenser
Transmission halogen light path
Reflection fluorescence light path
Fluorescence Filter Cubes:
8-position Motorized Turret
DAPI (UV) - EX: 360/40nm DM: 400lp EM: 460/50nm
FITC (Green) - EX: 470/40nm DM: 505lp EM: 535/50nm
Filter set 15 (Red)- EX: 546/12nm DM: 580nm EM: 590lp
12V 100W Halogen Lamp w/ built-in power supply
HBO100 Mercury Lamp w/ ebq100 power supply
Optiscan Motorized XY Stage
Optiscan Control Box
6-position Motorized Turret
10x/0.30 Plan-NEOFLUAR Ph1
*lenses may be visually different than seen in pictures and videos, but will have exactly these parameters
Trinocular Eye 100% or 100% Camera 50/50% split
AmScope MU300 CMOS 3.1MP Digital Color Camera
Computer and Software:
Dell Latitude i5 running Windows 10 with AmScope and MicroManager software
Objective Turret, Fluorescence Turret, Z-axis, Fluorescence Shutter, Condenser, XY Stage
Communication and power cables.
Configuration and Setup:
Illumination Type: Brightfield w/ Fluorescence, Phase Contrast
Objectives: 10x/0.30 Plan-NEOFLUAR Ph1, 20x/0.50 Plan-NEOFLUAR
Light Sources: 12V 100W Halogen Lamp, HBO100 Mercury Lamp
Stage: Motorized XY
This Zeiss Axioplan 2ie Mot Fluorescence Motorized Phase Contrast Microscopeis currently set up for Brightfeild with Phase Contrast and Fluorescence studies.
The AmScope MU300 CMOS digital camera is capable of taking detailed digital images.
This Zeiss system includes a Dell i5 Laptop running Windows 10 with AmScope software and MicroManager software.
The 10x eyepieces along with the 10x and 20x objectives give maximum magnification 200x.
This video beings with a full front view of the Zeiss Axioplan 2ie Mot Fluorescence Motorized Phase Contrast Microscopepowered up and running.
The camera pans from right to left for a closer look. We see the MicroManager software information and its control of the motorized components of the scope and stage.
Next we see the base of the scopes motorized control. Lastly we see the fluorescence lamp on and the filter cubes moved into the light path.
A final front view of the Zeiss Axioplan 2ie Mot Fluorescence Motorized Phase Contrast Microscopewill end this video.
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