Setup for Brightfield with Phase Contrast and Fluorescence studies.
This Zeiss Axioplan 2ie Fluorescence Phase Contrast Microscope is in good working order.
Microscope optics have been professionally cleaned under a class 10,000 clean room hood.
Please see the exact video and photos below.
Testing we performed:
The Zeiss Axioplan 2ie Fluorescence Phase Contrast Microscope passed all tests and was found to be fully functional:
Tested halogen lamp and source: PASSED
Tested mercury lamp and source: PASSED
Tested objective for clarity: PASSED
Tested port splitter to split between camera and eyepieces: PASSED
Tested condenser and prisms: PASSED
Tested mechanical stage to limits: PASSED
Tested mechanical Z-axis: PASSED
Tested nosepiece: PASSED
Tested fluorescence slider: PASSED
Tested connection from camera to laptop: PASSED
Tested live feed for clarity: PASSED
Tested all filter slides: PASSED
Tested all switches and adjustment knobs to be working fully: PASSED
During testing this Zeiss Axioplan 2ie Fluorescence Phase Contrast Microscope was found to have no issues.
A clear image was produced through the eyepieces as well as through the camera port.
All of the mechanical components moved freely and the test sample was able to be focused without excessive effort.
System consists of:
Zeiss Axioplan 2ie Upright Microscope
(2) PI 10x/25 Eyepieces
Prisms / Condenser:
Prisms: Ph1, Ph2
HAL100 12V 100W Halogen Lamp with built-in power supply
HBO100 Mercury Lamp with FluoArc power supply
Mechanical XY (60 x 80mm travel)
6-position Mechanical Nosepiece
10x/0.30 Plan NEOFLUAR
40x/0.65 Plan ACHROMAT
Head 100% or camera 100% or 50/50% split
AmScope MU300 3MP Digital Camera
Computer and Software:
Dell Latitude i5 running Windows 7 Pro woth AmScope software
Communication and power cables.
Configuration and Setup:
Illumination Type: Brightfield with Phase Contrast, Fluorescence
Objectives: 4x/0.10 ACHROPLAN, 10x/0.30 Plan NEOFLUAR, 40x/0.65 Plan ACHROMAT
Light Sources: HAL100 12V 100W Halogen lamp, HBO100 Mercury Lamp
Stage Type: Mechanical XY
This Zeiss Axioplan 2ie Fluorescence Phase Contrast Microscope is currently set up for Brightfield with Phase Contrast and Fluorescence studies.
This Zeiss Axioplan 2ie Fluorescence Phase Contrast Microscope is to be used for live cell and biological studies.
The AmScope MU300 CMOS digital camera is capable of taking detailed digital images.
This Zeiss system includes a Dell i5 Laptop running Windows 7 with AmScope software.
With eyepieces of 10x and objectives of 10x, 20x, 40x the total magnification for this microscope is 400x.
This video begins with a full front view of the Zeiss Axioplan 2ie Fluorescence Phase Contrast Microscope powered up and running.
The camera pans from one side to the other for a closer look. Next, we see the AmScope software information and a demonstration of the live feed by using the mechanical focus and XY.
We last see the fluorescence before a final front view of the Zeiss Axioplan 2ie Fluorescence Phase Contrast Microscope will end this video.
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