Seller assumes all responsibility for this listing.
Condition: Used - Excellent
Model: IX50 Microsc
Arcturus PixCell IIe Laser Microdissection
With Olympus IX50 Microscope
Includes UPlanFl 4x/0.13, UPlanFl 10x/0.30, LCPlanFl 20x/0.40, LCPlanFl 40x/0.60
Eyepieces are WH10X/22
Olympus Optical LampHouse U-LH100L-3 w/ Power Supply TH4-200 Lamp House
ARCTURUS ENGINEERING PIXELL IIe LASER CAPTURE MICROSCOPE COME WITH OLYMPUS IX50 MICROSCOPE ( BOTH IN LIKE NEW CONDITION )
Laser Capture Microscopy
The Advanced Microscopy & Imaging core facility houses a PIXCELL IIe laser capture microscope (Arcturus Engineering) that can be used to microdissect small clusters of cells, or even single cells, for use in DNA, RNA or protein analyses. Cells can be dissected from embedded tissue sections that have been stained with H&E, a special stain, or even an immunostain.
Laser Capture Microscopy Procedure: The procedure is simple and rapid. Training time is less than one hour, and a trained user can dissect from one to several thousand cells within a matter of minutes.
The PIXCELL II scope has an integrated digital image capture unit, allowing one to record "before" and "after" images of the dissected cells. In the example shown below, a pure population of tumor cells were dissected away from whole biopsy.
The Arcturus PixCell II Laser Capture Microdissection (LCM) microscope. This instrument is attached to an Olympus IX 50 and is equipped with a Sony DXC-151A Hyper HAD CCD/RIS/RGB camer with Sony camera adaptor CMA-D2 . This system is also used independently to capture images from H&E or HRP stained sections on glass slides . With this instrument one can quickly locate a single cell or large group of cells, technically aim-and-shoot and remove specific cells for subsequent molecular analysis. Regardless of whether one uses paraffin-embedded or frozen tissue samples, stained or immunolabeled slides, the LCM preserves the exact morphologies of both the captured cells as well as the surrounding tissue. The system is designed to easily monitor and document the entire process and stores images in the archiving workstation .
2003 model LCM1105; Current web price ±$42,500 (for a complete system)
The Arcturus PixCell IIe Laser Capture Microdissection system is an intuitive system for fast point-and-shoot microdissection. Cell capture with the PixCell IIe LCM system is direct and assured. Cells of interest can be directly picked up from the slide surface.
The laser capture micro-dissection microscope allows one to retrieve or "capture" a selected cell or group of cells from a stained or unstained paraffin or frozen section. Bio-molecules such as DNA, RNA, or protein can then be isolated from the captured cells for purification and analysis. Cells can also be captured from blood smears and from fine needle aspirates.
This unit is based on the Olympus IX50 Inverted Microscope. It powers on and looks like it's in beautiful condition. I don't have any slides or experience to test this fully, but it looks like it's in good shape. Has a 6-place turret. Only what is pictured is included in this listing.
One frequently used application of LCM is to obtain RNA from which cDNA is made by RT-PCR and then used for micro-array analysis to determine which genes are being expressed in the selected cells. Isolation of DNA from cells or from individual chromosomes from metaphase spreads can be used for genetic screening. Protein aggregates can be accumulated from intracellular vesicles for evaluation by western blot or amino acid Similarly, lipid droplets can be captured for subsequent analysis using a variety of biochemical techniques.
Laser capture microdissection (LCM) can be used to separate individual cell types from within complex tissues and organs. DNA or RNA can then be extracted from these microsections for gene expression analysis. LCM utilizes a low-power infrared laser to melt a special thermoplastic film over the cells or tissues of interest. Specifically developed caps that are coated with this thermoplastic film are placed on the tissue section of interest. The PixCell IIe LCM instrument is then used to direct the low-powered infrared laser through the cap to melt the film onto the cells of interest. These cells adhere to the cap when it is lifted from the tissue section – thus, capturing them for further analysis.
The System come with Pixcell II power supply and All necessary Cables, Power Cords .