Nikon Ti-U Inverted Microscope Fluorescence Phase Contrast
Setup for Brightfield Transmitted with Phase Contrast and Fluorescence Reflective studies.
This Nikon Ti-U Inverted Microscope is in good working order with all aspects tested to be working fully.
Microscope optics have been professionally cleaned under a class 10,000 clean room hood.
Please see the exact video and photos below.
Testing we performed:
The Nikon Ti-U Inverted Microscope passed all tests and was found to be fully functional:
Tested XY stage to limits: PASSED
Tested all microscope buttons: PASSED
Tested metal halide fluorescence light source: PASSED
Tested fluorescence light path and filters: PASSED
Tested halogen lamp and source: PASSED
Tested halogen lightpath and illumination arm: PASSED
Tested the port splitter to switch between 4 ports: PASSED
Tested connection from computer to camera and microscope: PASSED
Tested Z-axis adjustment and focus: PASSED
Tested live feed to computer: PASSED
Tested filters and prisms: PASSED
Tested all components of both lightpaths: PASSED
During testing this Nikon Ti-U Inverted Microscope was found to have no issues.
A clear image was produced through the eyepieces as well as through the camera port.
All of the mechanical and motorized components moved freely and the test sample was able to be focused without excessive effort.
System consists of:
Nikon Ti-U Inverted Microscope
Ti-TERG Ergonomic Binocular Head
(2) CFI 10x/22 Eyepieces
Prisms / Condensers:
ELWD 0.3 Phase Contrast Condenser
Prisms: PhL, Ph1, Ph2
Ti-DH Illumination Arm
Ti-FL Fluorescence light path with manual shutter
C-HGFL Metal Halide Illumination with C-HGFLB Culminating Adapter and liquid light guide
Ti-FLC 6-position Motorized Filter Cube Turret
UV-2E/C EX: 360/40nm DM: 400nm EM: 460/50nm
FITC EX: 480/40nm DM: 505nm EM: 535/50nm
TR HQ EX: 560/55nm DM: 595nm EM: 645/75nm
D-LH/LC 12V 100W Halogen Lamp with Ti-PS 100W Power Supply
C-HGFL Metal Halide Lamp with C-HGFLB Culminating Adapter and liquid light guide
Ti-Sr Mechanical XY Stage
Ti-ND6-E Motorized Nosepiece
10x/0.30 Plan Fluor Ph1 DL
20x/0.45 S Plan Fluor ELWD Ph1 ADM
40x/0.60 S Plan Fluor ELWD Ph2 ADM
Photo / Port Slits:
Left 100% or Right 100% or Head 100%
AmScope MU1003 10MP USB 3.0 CMOS color camera
Computer and Software:
Dell Latitude i5 running Windows 7 Pro with AmScope software
Communication and Power Cables:
(2) Standard 115V Power Cord
Ti-PS100W Control Cable
Configuration and Setup:
Illumination Type: Brightfield Transmitted w/ Phase Contrast, Fluorescence Reflective
Objectives: 10x/0.30 Plan Fluor Ph1 DL, 20x/0.45 S Plan Fluor ELWD Ph1 ADM, 40x/0.60 S Plan Fluor ELWD Ph2 ADM
Light Sources: 12V 100W Halogen Lamp, 100W Metal Halide Lamp
Stage: Mechanical XY
This Nikon Ti-U Inverted Microscope is currently set up for Brightfield Transmitted with Phase Contrast and Fluorescence Reflective studies.
The purpose of this microscope is to view biological studies. This Nikon Ti-U Inverted Microscope is capable of viewing slides, petri dishes and multi well plates.
The AmScope MU1003 10MP USB 3.0 colored CMOS camera is capable of taking detailed digital images and simple measurements.
The Nikon Ti-U Inverted Microscope system includes a Dell Latitude i5 Laptop to control camera software.
The microscope with included objectives of 10x, 20x and 40x plus the 10x eyepieces and included 1.5x internal magnification the maximum magnification is 600x.
The video starts off with a full view of the entire Nikon Ti-U Inverted Microscope. The camera pans around the system bringing into focus all included components.
The live feed is shown as the sample moved around and comes in and out of focus. The fluorescence shutters are opened and the halogen lamp is turned off.
The sample is illuminated from the fluorescence lamp and filter cubes.
The shutters are closed and the halogen lamp is turned back on and there is one final view of the entire Nikon Ti-U Inverted Microscope.
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