Here is a Nikon TE2000-U Inverted Fluorescence Phase Contrast Microscope Pred Ti Unit4
Setup for Brightfield with Phase Contrast and Fluorescence studies.
This Nikon TE2000-U Inverted Fluorescence Phase Contrast Microscope is in good working order with all aspects tested and working fully.
Microscope and optics have been professionally cleaned under a class 10,000 clean room hood.
Please see the exact video and photos below.
Testing we performed:
The Nikon TE2000-U Inverted Fluorescence Phase Contrast Microscope passed all tests and was found to be fully functional:
Tested halogen lamp and source: PASSED
Tested mercury lamp and source: PASSED
Tested port splitter to split between all ports: PASSED
Tested connection from computer to camera for live feed: PASSED
Tested mechanical XY stage: PASSED
Tested objectives for clarity: PASSED
Tested eyepieces for clarity: PASSED
Tested live feed for clarity: PASSED
Tested phase contrast condenser and light rings: PASSED
Tested fluorescence light path and shutters: PASSED
Tested fluorescence filter cubes and turret: PASSED
Tested all power switches and light adjustments on microscope: PASSED
During testing this Nikon TE2000-U Inverted Fluorescence Phase Contrast Microscope was found to have no issues.
A clear image was produced through the eyepieces as well as through the camera port.
All of the mechanical components moved freely and the test sample was able to be focused without excessive effort.
System consists of:
Nikon Eclipse TE2000-U Inverted Microscope
T-TD Binocular Tube Head
(2) CFI 10x/22 Eyepieces
Prisms / Condensers:
ELWD 0.3 Condenser
Prisms: PhL,Ph1, Ph2
T-DH Illumination Tower
Fluorescence Light Path:
T-FL w/ Manual Shutters
Fluorescence Filter Cubes:
T-FLC 6-position Turret
UV-2A- EX: 355/50nm DM: 400nm EM: 410nm
GFP- EX: 470/40nm DM: 495nm EM: 525/50nm
TxRED- EX: 562/40nm DM: 592nm EM: 624/40nm
LHS-H100P-1 Halogen 12V 100W Lamp Housing with TE-PS100 power supply
LH-M100C-1 100W Mercury Lamp with C-SHG1 power supply
Manual 6-position Objective Turret
4x/0.2 Plan Apo
10x/0.30 Plan Fluor Ph1 DL
20x/0.75 CFI Plan Apo VC (this is a high quality Nikon APO CFI60 Objective, it was originally installed in a custom piece of equipment where manufacturer did not want to show that they used Nikon objectives)
Photo / Port Splits:
100% Eye or 100% Left Port or Right 80% Eye 20% or Front 80% Eye 20%
AmScope MU1003 10MP Digital Color Camera USB 3.0
Computer and Software:
Dell Latitude i5 running Windows 10 with AmScope software
Communication and power cables.
Configuration and Setup:
Illumination Type: Brightfield w/ Fluorescence and Phase Contrast
Objectives: 10x/0.30 Plan Fluor Ph1 DL, 20x/0.75 CFI Plan Apo VC, 40x/0.75 Plan Fluor DIC M/N
Light Sources: 12V 100W Halogen Lamp, 100W Mercury Lamp
Stage Type: Mechanical XY
This Nikon TE2000-U Inverted Fluorescence Phase Contrast Microscope is currently set up for Brightfield studies with Phase Contrast and Fluorescence.
The MU1003 10MP AmScope CMOS digital color Camera is capable of taking detailed digital images and simple measurements of samples.
The Nikon TE2000-U Inverted Fluorescence Phase Contrast Microscope system includes a Dell Latitude i5 Laptop to control the AmScope software for live images.
This video begins with a full front view of the Nikon TE2000-U Inverted Fluorescence Phase Contrast Microscope powered up and running.
The camera pans from right to left for a closer look at detail. Next, we see the AmScope software live feed imaging.
A demo of the live feed is shown using the mechanical focus as well as XY axis'. We then see the Fluorescence to be working with the naked eye.
A final front view of the Nikon TE2000-U Inverted Fluorescence Phase Contrast Microscope will end this video.
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