Here is a Nikon TE2000-E PFS Perfect Focus Motorized Fluorescence DIC Polarization Phase Contrast Microscope
Setup for Brightfield Transmitted with Phase Contrast, Polarization, DIC and Fluorescence Reflective studies
This Nikon TE2000-E is also setup with Perfect Focus System (PFS)
Ti / Ti2 series
This Nikon TE2000-E PFS Motorized Fluorescence DIC Polarization Phase Contrast Microscope is in good working order with all aspects tested and working fully.
Microscope optics have been cleaned professionally by our technician under a class 10,000 clean room hood.
Please see the exact video and photos below.
Testing we performed:
The Nikon TE2000-E PFS Motorized Fluorescence DIC Polarization Phase Contrast Microscope passed all tests and was found to be fully functional:
Tested control of motorized XY stage to limits: PASSED
Tested joystick buttons and controls fully: PASSED
Tested control of all motorized components including Z-axis focus, XY motorized stage, lightpath switching and the PFS system: PASSED
Tested all microscope buttons to works controlling motorized components: PASSED
Tested control of microscope with computer software: PASSED
Tested fluorescence system and lightpath to be fully working: PASSED
Tested halogen lamp, source and lightpath to be fully working: PASSED
Tested the motorized port splitter to switch between all ports: PASSED
Tested connection from computer to camera and microscope: PASSED
Tested live feed to computer: PASSED
Tested filters and prisms: PASSED
Tested all components of both light paths to be working fully: PASSED
Tested PFS system to be working fully: PASSED
Tested eyepieces for clarity: PASSED
Tested objectives for clarity: PASSED
During testing and cleaning this Nikon TE2000-E PFS Motorized Fluorescence DIC Polarization Phase Contrast Microscope was found to have no issues.
A clear image was produced through the eyepieces as well as through the camera port.
All of the mechanical components moved freely and the test sample was able to be focused without excessive effort.
System consists of:
Nikon TE2000-E Inverted Microscope
TE-TD Binocular Head
(2) CFI 10x/22 Eyepieces
Prisms / Condensers:
5-position Condenser Head LWD 0.52 Lens
Prisms: DIC N2, Ph1
Analyzer / Polarizer:
T-DH Illumination Arm
T-FL Fluorescence Light Path
C-HGFIA Culminating adapter
EXFO X-Cite 120 Metal Halide light source w/ liquid light guide
T-FLC 6-position Mechanical Filter Cube Turret
ONE D/F/R triband filter cube with specs below.
DAPI- EX: 350/50nm DM: 400lp EM: 470/40nm
FITC- EX: 470/40nm DM: 500lp EML 525/40nm
Tx RED- EX: 560/55nm DM: 595lp EM: 645/75nm
DIC N2 Condenser Prism
40x/0.90 S Plan Fluor DIC N2
D-LH 12V 100W Illumination arm
LHS-H100P-1 Halogen Lamp with TE2-PS100W Halogen Power Supply
EXFO X-Cite 120Q Metal Halide Lamp supply with C-HGFIA Culminating adapter
PRIOR H30V4 Controller
PRIOR CS152DP Joystick
PRIOR H117P2TE Motorized Stage XY
Perfect Focus System:
T-PFS Main Optical Unit
T-PFC Main Controller
6-position Mechanical Nosepiece
10x/0.30 Plan Fluor Ph1 DL
20x/0.75 CFI Plan Apo VC (this is a high quality Nikon APO CFI60 Objective, it was originally installed in a custom piece of equipment where manufacturer did not want to show that they used Nikon objectives)
40x/0.90 S Fluor DIC N2 with 40x PF slider
Photo / Port Slits:
Left 100% or Left 80% Head 20% or Right 100% or Head 100%
AmScope MU1003 10.0MP USB 3.0 Colored CMOS camera
XY Stage, Z-Axis, Light Path switching, Perfect Focus system
Computer and Software:
Dell Latitude i5 running Windows 7 Pro with AmScope and MicroManager software
Communication and power cables:
(3) Standard 115V Power Cord
Power Adapter for Nikon Ti-Base
TE2-PS100W Control Cable
Motorized XY stage Cable
(2) RS232 communication cable
Configuration and Setup:
Illumination Type: Brightfield w/ Fluorescence, Phase Contrast, DIC and Polarization
Objectives: 10x/0.30 Plan Fluor Ph1 DL, 20x/0.75 CFI Plan Apo VC, 40x/0.90 S Fluor DIC N2
Light Sources: 12V 100W Halogen Lamp, EXFO X-Cite 120 Metal Halide Lamp
Stage: Motorized XY
This Nikon TE2000-E PFS Motorized Fluorescence DIC Polarization Phase Contrast Microscope is currently set up for Brightfield transmitted with Phase Contrast, Fluorescence, DIC and Polarizartion studies.
This Nikon TE2000-E PFS Motorized Fluorescence DIC Polarization Phase Contrast Microscope has the PFS (Perfect Focus System) installed which holds focus irregardless of temperature fluctuation or vibrations.
The purpose of this Nikon TE2000-E PFS Motorized Fluorescence DIC Polarization Phase Contrast Microscope is to view biological studies.
This Nikon TE2000-E PFS Motorized Fluorescence DIC Polarization Phase Contrast Microscope is capable of viewing slides, petri dishes, and multi well plates.
The AmScope MU1003 10MP CMOS digital camera is capable of taking detailed digital images and making simple measurements.
The Nikon TE2000-E PFS Motorized Fluorescence DIC Polarization Phase Contrast Microscope system includes a Dell Latitude i5 laptop to control the stage and microscope.
This laptop includes AmScope live feed and MicroManager software. With included objectives of 10x, 20x, 40x plus the 10x eyepieces and included 1.5x internal magnification the maximum magnification of this microscope is 600x magnification.
This video beings with a full front view of the Nikon TE2000-E PFS Motorized Fluorescence DIC Polarization Phase Contrast Microscope system powered up and running.
The camera pans from right to left for a closer look. We see the MicroManager software and the AmScope live feed.
Next, we see control of the XY prior stage and the motorized focal adjustment virtually and physically. We then see the fluorescence as it illuminates the sample.
A final front view of the Nikon TE2000-E PFS Motorized Fluorescence DIC Polarization Phase Contrast Microscope system will end this video.
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