For Sale Zeiss AXIO Observer Z1 Inverted LED Fluorescence Motorized XY with Definite Focus Microscope (New Filters) Pred Observer 7
Pre-owned microscope (this microscope can be fully customized)
• Fully Functional
• Serviced & in Stock
• Good cosmetic condition
• Comes with Brand New Fluorescence Filter Sets
• Continental US Warranty 120 Days & 60 Days International.
• The training package is available.
• Renewable single or multi-year warranty agreements are available.
The Predecessor to Zeiss Observer 7 Inverted Fluorescence Microscope
This is what you will receive before customizing this microscope:
Standard Zeiss AXIO Observer Z1 LED Fluorescence Microscope with Definite Focus Configuration:
Microscopy Techniques:
Brightfield and Fluorescence Microscopy
Microscope Base:
• Zeiss Observer Z1 Base with camera port
• Motorized(Automated) Z-Axis Drive
• With Zeiss Touch Controller
Microscope Head:
• Binocular Head
• Two Eyepieces
Fluorescence Light Path:
• Zeiss fluorescence lightpath built into the base
Fluorescence Illumination:
• Lumencor LED Aura 5 Color Light Engine
• Light guide
• Colliminating adapter
Fluorescence filter cube turret:
• Motorized 5-position filter cube turret
Fluorescence filters:
• Three filter cubes
• Three Brand New filter sets of either DAPI, FITC, Texas Red, CY5, CY3
Brightfield Light Path:
• Illumination Tower
Brightfield Illumination:
• 100 Watt halogen light source
• 100 Watt halogen power supply built into the base
Objective Turret:
• Motorized 6-position objectives turret
• Zeiss Definite Focus Drift System with Controller
Objectives:
• 5x/0.15 Plan-Neofluar
• 10x/0.30 Plan-Neofluar
• 20x/0.40 Plan-Neofluar
Condenser:
• Motorized condenser
Stage:
• Motorized Stage XY
• Controller
• Joystick
Camera ports:
• 100% Eyepiece, 0% Camera
• 20% Eyepiece, 80% Camera
• 0% Eyepiece, 100% Camera
Camera adapter:
• 1x C-mount adapter
Camera:
• CMOS USB 3.0 10MP Camera
Computer & Software:
• Dell laptop computer with Windows 10
• Micromanager w/ImageJ analysis software
• Camera software
Accessories:
• Necessary power and communication cables
This configuration includes any 3 of these new filter sets.
• New filter set - DAPI (EX: 365/28nm, DM: 405nm, EM: 445/50nm) (Dye compatibility: DAPI/Hoechst/AlexaFluor 350)
• New filter set - EGFP/FITC/Cy2 (EX: 475/30nm, DM: 500nm, EM: 530/40nm) (Dye compatibility: EGFP/FITC/Cy2/AlexaFluor 488)
• New filter set - TRITC/Cy3/Dsred (EX: 545/20nm, DM: 562nm, EM: 605/60nm) (Dye compatibility: TRITC/Cy3/RFP/Rhodamine/AlexaFluor 546)
• New filter set - Texas Red (EX: 560/20nm, DM: 585nm, EM: 630/40nm) (Dye compatibility: Texas Red/mCherry/AlexaFluor 594)
• New filter set - Cy5 (May require an upgraded light source) (EX: 630/50nm, DM: 662nm, EM: 695/50nm) (Dye compatibility: Cy5/AlexaFluor 647/Draq5)
Automated/motorized capabilities of this microscope:
Installed automation/motorized components:
• Motorized lightpath
• Motorized/automated Z-Axis Drive
• Motorized filter cube turret
• Motorized objective(nosepiece) turret
• Motorized Condenser
• Motorized XY Stage
• Definite Focus Drift Control System
Benefits of motorized/Automated Z-Axis drive:
• Automatic parfocality adjustment
• The ability to recall previously saved focus levels
• Automated management of Z positioning/ Fine-coarse control
How the Nikon Perfect Focus System Works
• Infrared Light Technique: Infrared light that doesn't affect the specimen is used to detect focal shifts.
• Real-Time Monitoring: Continually checks and adjusts the focal plane to maintain sharp imaging.
• Sensor-Based Feedback: Employs sensors to measure deviations from the desired focus point.
• Automated Corrections: Adjusts the position of the microscope Z-axis to correct any focus drift automatically.
• Integration with Imaging Software: Works in tandem with microscope software for seamless operation.
• Hardware Compatibility: Designed to integrate effortlessly with a variety of Zeiss microscope systems.
Benefits of Zeiss Definite Focus System:
• Enhances Image Quality: Provides sharp, clear images by maintaining constant focus.
• Reduces Need for Re-Calibration: Saves time and improves efficiency in long-term experiments.
• Optimized for Live-Cell Imaging: Allows continuous observation without disturbing delicate samples.
• Compatible with Various Microscopes: Easily integrates with a range of Zeiss microscope models.
• Automatic Adjustments: Continuously compensates for focus drift during imaging sessions.
• User-Friendly: Simple to set up and operate, even for novice users.
• Boosts Productivity: Enables longer uninterrupted imaging sessions without manual intervention.
• Improves Data Consistency: Yields reliable and reproducible results across multiple experiments.
• Supports Complex Experiments: Facilitates advanced techniques like time-lapse microscopy.
• Minimizes Manual Effort: Reduces the workload on researchers by automating routine tasks.
• Versatile Application Range: Suitable for research in fields like oncology, neurobiology, and developmental biology.
• Robust Design: Built to withstand rigorous use in professional laboratory environments.
This microscope's imaging capabilities:
• Ability to take still images and live videos of your samples
• Display, annotate, edit, calibrate, measure, analyze, process, print, and save raster
• Variable linear measurements such as line segment, radius values, and others
• Open source Micromanager Multi-dimensional Image acquisition & automation software capabilities
• Multi-frame (Time lapse/Image acquisition over time)
• Multi-slice (Z coordinate/Z Stacking)
• *Multi-position (XY coordinate/Stitching/Mosaic) – only available when equipped with a motorized XY Stage
We can customize this microscope to fit your requirements:
Add Microscopy Techniques (In addition to installed Brightfield and Fluorescence)
• Phase Contrast, DIC, or Polarization Add-on Components may be added (subject to availability)
Increase Maximum Magnification and Image Quality:
• Microscope can be configured for magnification between 40x and 1000x (with 10x Eyepieces)
• We can add standard higher magnification Zeiss microscope objectives
• We can add high-end objectives
Enhance fluorescence capabilities by adding/changing filters and upgrading light sources.
• Additional filter cubes
• Additional filter sets to match your fluorochromes.
• Xenon, Metal Halide, or LED Fluorescence microscope light sources
We will also ensure the fluorescence light source matches your selected filter sets.
Enhance imaging capabilities:
• We offer various advanced CCD, sCMOS, and EMCCD camera options for higher-quality images.
Add other Motorized/Automation Capabilities:
In addition to installed motorized capabilities, the microscope can be configured
• Motorized high-speed shutter
We can configure it to work with the sample medium you need:
• Slide, Petri dish, microplate, etc.
• It may also be set up with a motorized XY stage for multi-slide or full microplate scans.
Commonly Requested Customer Upgrades
• High-End CCD, sCMOS Cameras
• LED or Metal Halide Fluorescence Light Sources
• Phase Contrast, Polarization, or the DIC configurations
• Higher Magnification Objectives
• Higher performance Fluor or APO objectives
• Motorized XY Stage (stage, controller, joystick)
We are excited to help build and configure the right microscope for you!
Zeiss AXIO Observer Z1 LED Fluorescence Definite Focus Microscope Overview
(Final microscope capabilities will depend on the modifications you request)
Suitable for the following applications as configured:
• Advanced Inverted Microscope: Optimized for detailed live cell imaging in high-end research settings.
• Definite Focus System: Automatically maintains sharp focus to prevent drift during long-term experiments.
• Tailored for Cell Biology: Excellently suited for complex neuroscience and developmental biology studies.
• Infrared Focus Technology: Non-invasive infrared technology is used to stabilize the focal plane, preserving sample integrity.
• Continuous Imaging: Ideal for extended observations without the need for frequent manual adjustments.
• Increases Research Productivity: Reduces manual intervention, allowing researchers to focus on analysis.
Other potential applications:
• Phase contrast and DIC: Enhances visibility of transparent specimens without staining.
• Time-lapse photography: Captures sequential images to monitor changes over time.
• Multi-dimensional imaging: Allows for the acquisition of images in multiple planes and dimensions.
• Quantitative analysis: Supports measurements and quantification of specimen features.
• High-resolution imaging: Provides clear, detailed images for in-depth analysis of samples.
• Live cell observation: Enables detailed study of live cell dynamics in real-time.
Why should you buy from us?
Our Advantage
• We make sure to match the microscope capabilities to your requirements.
• We have experience servicing and repairing these microscopes.
• This microscope goes through an extensive set of service steps before shipping.
• We offer detailed photos and videos of the microscopes you buy.
• We are an approved pre-owned equipment vendor to many large US, Canadian, and EU Universities.
• We are an approved pre-owned equipment vendor to many Fortune 500 US and International Biopharma companies.
• Many upgrade and repair parts are in stock.
• Remote installation and microscope operation training are available.
• On-site installation may be available in the Boston Area (New England area)
• Expert US or International shipping is available (see the shipping tab on how we pack and feel at ease)
Our Guarantee
• It will work!
• Continental US Warranty 120 Days & 60 Days International.
• An extended warranty may be available.
• Renewable single or multi-year warranty agreements are available.
• You get after-sales technical support.
• Smooth process from the initial quote to order fulfillment.
• Customer references are available.
*SM* Small Crate
200#
PLEASE NOTE QUESTIONS BELOW ARE ANSWERED IN MORE THOROUGH DETAIL ON OUR WEBSITE
Q: I am not 100% sure about the configuration I need, can your team assist me with selecting the right microscope for me?
A: Absolutely, we can start with your application, samples, or challenge to assist you in selecting the right configuration and the right microscope for you.
Q: Can you make the necessary modifications to the standard configuration to fit my application?
A: Certainly, we carry a large variety of upgrade components to ensure the final microscope configuration will fit your application
Q: What steps do you take to verify, service, and confirm the functionality of this Microscope?
A: Each microscope goes through extensive service to ensure it performs to specification.
Q: Can I view this microscope in person at your facility or do a live Zoom demo before it is shipped to me?
A: We can accommodate both in-person or Zoom demos.
Q: What about the warranty?
A: The microscope comes fully warranted for both parts and labor.
Q: Do you offer installation, service, etc?
A: We offer remote installation and training packages to get you familiar with this microscope
Q: What should I watch out for when buying a used/pre-owned microscope from another lab?
A: That it has no missing, broken, or incorrect parts for your application, any mechanical or motorized components have been serviced and lubricated, controller issues, good quality filter cubes, proper and undamaged objectives, and most importantly no dust in the light path or objectives.
Q: Do you have these and other microscopes on hand?
A: We carry this and many other microscopes from Nikon, Zeiss, Olympus, and Leica.
Q: I am from the University or College, do you accept Purchase Orders?
A: We accept purchase orders from all major US and Canadian universities.
Q: How fast will you ship?
A: We can ship within 2-3 days to about 2 weeks, depending on the final configuration required.
Q: Will my instrument be insured during shipping?
A: Absolutely! We insure all our shipments as long as we are the shipper. If a customer ships on their account, they handle insurance.
Q: Have you packed and shipped microscopes in the past?
A: We have extensive experience in safely shipping high-end and complex microscopes all over the world.
Q: Do you ship Internationally?
A: So far, we have shipped to almost every continent and over 30 countries.
Q: How much will I save buying it pre-owned, and is it worth it?
A: When compared to buying a new one, you will save up to 60% to 80% and have plenty of funds left over for an additional instrument or two.
TO VIEW ADDITIONAL PHOTOS, VIDEOS AND VERY DETAILED INFORMATION SUCH AS TEST STEPS PERFORMED, CONFIGURATION, ETC., PLEASE VISIT OUR WEB SITE. TO GET THERE PLEASE CLICK ON “WEBSTORE”; TO YOUR RIGHT AND YOU WILL SEE LINK TO OUR SITE IN THE MIDDLE OF THE SCREEN. THEN USE LAST 5 DIGITS OF MPN NUMBER IN THE SEARCH FIELD TO LOCATE YOUR ITEM.
DO NOT HESITATE TO CALL US OR EMAIL US WITH ANY QUESTIONS YOU MIGHT HAVE.
ALL OF OUR ITEMS ARE AVAILABLE FOR INSPECTION AT OUR FACILITY.
Return Policy
Items are sold as-is with no returns or refunds available unless explicitly stated.
