Here is a Nikon Electrophysiology Rig Patch Clamp Ti-S Fluorescence and DIC Microscope w/ Sutter Micromanipulator, HEKA EPC 10 Patch Clamp, Faraday Cage
Setup for Patch Clamp Electrophysiology, Brightfield with DIC, and Fluorescence studies with Brand New Fluorescence Filters
- This setup includes a Sutter MP-285 micromanipulation system with HEKA 10 headstage
- The headstage allows for recording using Heka Patch Master software
- A Faraday cage and TMC Antivibration table are included for improved sample recording
- This microscope is optimized for observation of biological studies where enhanced contrasting methods are necessary.
- Enhancing components, such as Fluorescence Filter Cubes have been added (Other components are available)
- The total current magnification range of this microscope is 40 to 200x (Other objectives are available)
- The fluorescence light source included is Metal Halide light source
- The XY mechanical stage has a travel range of 70 x 50mm. It will allow for observation of prepared slides and petri dishes.
- The included TMC Anti-Vibration features TMCs Gimbal Piston Air Isolators, allowing quick dissipation of disturbances of the isolated table top and clear imaging without interference from local vibration around the lab.
Predecessor to:
Current model
Condition:
This Nikon Electrophysiology Rig Patch Clamp Ti-S Fluorescence and DIC Microscope is in good working order with all of its aspects tested and fully functional
Optics Inspection:
Microscope and optics have been professionally cleaned by our microscopy technical team.
This inspection is repeated prior to the microscope being packed for shipping to its new destination.
Testing we performed:
The Nikon Electrophysiology Rig Patch Clamp Ti-S Fluorescence and DIC Microscope passed all tests and was found to be fully functional:
Tested LED Lamp and source fully: PASSED
Tested Metal Halide source fully: PASSED
Tested transmission light path: PASSED
Tested objectives for clarity: PASSED
Tested port-splitter to split between camera and eyepieces: PASSED
Tested mechanical condenser: PASSED
Tested mechanical stage: PASSED
Tested mechanical Z-axis: PASSED
Tested mechanical objective turret: PASSED
Tested connection from camera to software: PASSED
Tested live feed for clarity: PASSED
Tested Sutter MP-285 MicroManipulator: PASSED
Tested HEKA EPC 10: PASSED
Verified live reading with software: PASSED
Perform "Full Test" function with PatchMaster software: PASSED
During testing and cleaning this Nikon Electrophysiology Rig Patch Clamp Ti-S DIC Microscope was found to have no issues.
A clear image was produced through the eyepieces as well as through the camera port.
All of the mechanical components moved freely and the test sample was able to be focused without excessive effort.
System consists of:
Base:
Nikon Eclipse Ti-S Inverted Microscope
Faraday Cage
TMC Anti-Vibration Table
Head:
Ti-TS B Binocular Head
(2) CFI 10x/22 Eyepieces
Condenser:
LWD 0.52 Condenser
DIC NI Condenser Insert
Brightfield Path:
TI-DH Illumination Arm
Fluorescence Path:
TI-FL-EPI path
Fluorescence Filter Cubes:
6-position Motorized Turret
DAPI - EX 375/28nm DM 415nm EM 460/50nm (NEW FILTER SET)
FITC - EX 480/35nm DM 505nm EM 535/40nm (NEW FILTER SET)
Texas Red - EX 550/20nm DM 585nm EM 630/40nm (NEW FILTER SET)
*Other filters available - please inquire*
Illumination:
PRIOR Brightfield LED Illumination System LDB102NI
EXFO X-Cite 120 Metal Halide w/ collimating adapter and light guide
Stage:
Mechanical XY
Sutter Stage attachment
Micromanipulation:
Sutter MP-285 Micromanipulation
Sutter ROE285-2223 Micromanipulation Stage Controller
Amplification:
HEKA Patch Clamp EPC10 USB
Objectives:
6-position Mechanical Turret
4x/0.13 Plan Fluor
10x/0.30 Plan Fluor DIC L/N1
20x/0.75 Plan Apo VC (this is a high quality Nikon APO CFI60 Objective, it was originally installed in a custom piece of equipment where manufacturer did not want to show that they used Nikon objectives)
*Other objectives available - please inquire*
DIC Components/Polarization:
T-A2 Analyzer
T-P2 Polarizer
10x DIC prism
20xI DIC prism
Photo/Port Splits:
100% Eyepiece, 0% Camera
20% Eyepiece, 80% Camera
Camera:
10MP CMOS Camera
Computer and Software:
Computer running PatchMaster (v2x92) software w/dongle, and applicable camera software
Communication and power cables.
Configuration and Setup:
Orientation: Inverted
Illumination Type: Brightfield w/ DIC, Fluorescence
Objectives: 4x/0.13 Plan Fluor, 10x/0.30 Plan Fluor DIC L/N1, 20x/0.75 Plan Apo VC
Light Source: PRIOR Brightfield LED, EXFO Metal Halide
Stage: Mechanical XY
This Nikon Electrophysiology Rig Patch Clamp Ti-S DIC Microscope is currently set up for Fluorescence and Brightfield with DIC studies.
The CMOS digital color camera is capable of taking detailed digital images.
The Nikon system includes a computer to control the software for live images.
Video Description:
*This video shows the actual microscope in this listing.*
The video provides a walk around view of this Nikon Electrophysiology Rig Patch Clamp Ti-S DIC Microscope and its included components.
A functionality demonstration has been performed to show proper operation of the Nikon Electrophysiology Rig Patch Clamp Ti-S DIC Microscope.
Any available additional options functionality has also been demonstrated.
The camera that will be included with this microscope may differ from the one shown in the demonstration.
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Items are sold as-is with no returns or refunds available unless explicitly stated.
